Human Cardiac Troponin-C (Recombinant)

Human Cardiac Troponin-C (Recombinant)
Product Code
11052E
Stage / Grade
Highly Purified / Recombinant
R.M. Source
Expressed in E. coli
Concentration Range
0.5 - 10.0 mg/mL
Buffer Matrix & pH
150mM NaCl, 10mM sodium phosphate, 0.05% NaN3 (pH: 7.0)
Optimal Storage Temperature
-20 °C to -70 °C (avoid repeated freeze-thawing)

Quality Control Methods / Results

Protein % Purity Range
≥ 95%
Quality Methods Used
SDS PAGE: Single 18 kDa band
Functionally capable of binding to Troponin-I
Total Protein Method
Bradford Protein Assay vs. BSA as reference standard

Physical Characteristics

Shelf Life
5 years @ -25 °C or ↓
Filtration
No filtration

Troponin-C is one of the three subunits that form the troponin complex of the thin filaments of striated muscle.  Cardiac Tn-C is characterized by a molecular weight of ~18 kDa.  It is expressed in heart and slow twitch skeletal muscle.  Recombinant human cardiac Tn-C is purified from E. coli extracts using a combination of ion-exchange and affinity chromatography steps.

 

HAZARD TESTING & WARNINGS:

N/A

 

Use Statement: For Research, Remanufacturing, or Immunization use for products not subject to license under PHS Act Section 351 only.