Rabbit Skeletal Muscle Troponin Complex

Product Code

13300G

Stage / Grade

Highly Purified

R.M. Source

Alcohol denatured rabbit skeletal muscle (fast twitch)

Concentration Range

1.0 - 5.0 mg/mL

Buffer Matrix & pH

50mM NaCl, 10mM imidazole, 5mM β-mercaptoethanol, 0.1mM CaCl₂, 0.05% NaN₃ (pH 7.0)

Optimal Storage Temperature

-20 °C to -70 °C (avoid repeated freeze thawing)

Quality Control Methods / Results

Protein Quantitation Method

Concentration calculated assuming an extinction coefficient of 0.4142 at 280nm for a 1 mg/mL solution

Protein % Purity Range

≥ 90%

Quality Methods Used

SDS PAGE: Three bands corresponding to Tn-T, Tn-I, and Tn-C

Physical Characteristics

Clarity

Clear colorless solution

Shelf Life

5 years @ -25 °C or ↓

Filtration

No filtration

Troponin is a complex of three subunits (Troponin-C, Troponin-I, and Troponin-T) that is integral to muscle contraction in skeletal and cardiac muscle but not smooth muscle. Troponin complex is purified using a combination of affinity and ion-exchange chromatography steps.

HAZARD TESTING & WARNINGS:

N/A

Use Statement: For use in Research or Remanufacture of non-injectable products not subject to license under PHS Act Section 351 only.