Product Name ANIMAL SKELETAL MUSCLE TROPONIN - I
Product Codes 13110G :RABBIT
13210G : BOVINE
13310G : PIG
13410G : SHEEP
13510G :CHICKEN
Stage / Grade Highly Purified
R.M. Source Skeletal Muscle; Species specific
Protein Concentration 0.25 - 2.0 mg/mL (Bradford Assay vs. BSA)
Buffer Matrix & pH

8M Urea, 100mM NaCl, 50mM Tris.HCl, 1mM EDTA, 15mM ß-mercaptoethanol (pH: 7.4)

Storage Temperatures -20° C to -70°C

Quality Control Methods / Results

Analyses:

  • PURITY: > 95% by SDS PAGE
(1) Rabbit CODE # 13110G Single major 22kDa band corresponding to RbSkM Tn-I. (Bulk qty. available)
(2) Bovine CODE# 13210G ~ 22 & 24kDA bands of approx. equal intensity indicative of fast and slow twitch isoforms of skeletal muscle Troponin - I.
(3) Pig CODE# 13310G ~ 22 and 24kDa bands indicative of fast and slow twitch isoforms of skeletal muscle Troponin - I. Minor low molecular wt. Tn-I fragments may be apparent.
(4) Sheep CODE# 13410G Major 24kDa band. Minor low molecular wt. Tn - I fragments apparent.
(5) Chicken CODE# 13510G Major 22kDa band. Minor low molecular wt. TN - I fragments apparent.

Comments:

Purified by Tn-C affinity chromatography. Product is used to confirm lack of cross-reactivity of antibodies raised against human cardiac Tn-I or to remove antibodies cross-reactive with the cardiac isoform when linked to agarose.

Optimum Storage Conditions: -25° C or below (Avoid repeated freeze thawing).

HAZARD TESTING & WARNINGS:

N/A

 

Use Statement: For use in Research or Remanufacture of non-injectable products not subject to license under PHS Act Section 351 only.

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