Quality Control Methods / Results

Analyses:

• SDS PAGE: Major 52 kDa band with minor proteolytic fragments.
• Western Blot:Major 52 kDa Band with minor proteolytic fragments.
• Specific protein concentration determined by Bradford assay vs. BSA.

Comments:

When expressed in E. Coli., Ro-52 has a tendency to undergo proteolytic degradation. The proteolytic fragments remain associated during purification and all exhibit strong immunoreactivity against human anti-Ro/SS-A antiserum in Western Blots.

HAZARD TESTING & WARNINGS:

N/A

Use Statement: For use in Research or Remanufacture of non-injectable products not subject to license under PHS Act Section 351 only.