* Reactivity of mouse, rat, rabbit, and canine cardiac troponin-I was demonstrated by dilution of purified animal cardiac Tn-I (stabilized with rabbit troponin-C) into serum from the respective species. Experiments have shown that cardiac Tn-I from mouse and rat give linear responses to approximately 50 ng/mL. Cardiac Tn-I from rabbit and dog show linear responses to approx. 20 ng/mL. Please note that the Troponin-I ELISA Kit is sold for research purposes only and that the kit was designed for specific measurement of Human Cardiac Troponin-I.

The Troponin-I ELISA kit uses four monoclonal antibodies directed against distinct Troponin-I antigenic determinants. Three antibodies are used for solid phase immobilization (on microtiter wells). The fourth is conjugated to HRP and used for detection. The test sample is allowed to react simultaneously with the four antibodies resulting in Troponin-I being sandwiched between solid phase and HRP- linked antibodies. After a 90 minute incubation period at room temperature the wells are washed with water. A solution of H2O2/TMB is then added and the reaction stopped after 20 minutes by addition of 3N HCl. The absorbance value at 450 nM (or 405 nM) is determined using a 96-well microtiter plate reader. Human Cardiac Troponin-I concentration is directly proportional to A450 (or A405) and is determined by reference to human cardiac troponin-I standards (0, 2.0, 7.5, 30 and 75 ng/mL) supplied in lyophilized form with the kit.

Use Statement: For Research Use Only