Human Cardiac Myoglobin

Product Code

10580A

Stage / Grade

Highly Purified / Immunogen Grade

R.M. Source

Human Cardiac Tissue

Concentration Range

0.5 - 10.0 mg/mL

Buffer Matrix & pH

50% Glycerol, 150mM NaCl, 10mM sodium phosphate, 0.05% NaN₃ (pH 7.0)

Optimal Storage Temperature

-20 °C (do not store at lower temperatures)

Quality Control Methods / Results

Protein % Purity Range

≥ 98%

Quality Methods Used

SDS PAGE: Single 17 kDa band
Western Blot: Identity confirmed using goat anti-human cardiac myoglobin antisera

Total Protein Method

Bradford Protein Assay vs. BSA as reference standard

Physical Characteristics

Color

Slightly Red

Clarity

Clear

Shelf Life

5 years @ -20 °C

Filtration

0.2 μm filtered

Myoglobin is a single-chain heme containing protein with a molecular weight of ~17 kDa. It is the primary oxygen-carrying protein of muscle tissues. The same isoform is expressed in both cardiac and skeletal muscle. It is a sensitive biomarker for muscle injury and has been widely used as a marker of heart disease. Myoglobin is purified using a combination of gel-filtration and ion-exchange chromatography steps.

HAZARD TESTING & WARNINGS:

Blood samples from tissue donors used in the manufacture of this product tested negative for HBsAg, Anti-HIV-1/HIV-2, Anti-HTLV 1/2, anti-HepB core, Anti-Hepatitis C, and RPR/STS by FDA approved methods.

CAUTION: NO TEST CAN GUARANTEE A PRODUCT TO BE NON-INFECTIOUS. THEREFORE, ALL MATERIAL DERIVED FROM HUMAN FLUIDS OR TISSUES SHOULD BE CONSIDERED AS POTENTIALLY INFECTIOUS.

Use Statement: For Research, Remanufacturing, or Immunization use for products not subject to license under PHS Act Section 351 only.