Human Skeletal Muscle Troponin-I (Native)

Human Skeletal Muscle Troponin-I (Native)
Product Code
13050G
Stage / Grade
Highly Purified
R.M. Source
Human Skeletal Muscle
Concentration Range
0.5 - 2.5 mg/mL
Buffer Matrix & pH
8M Urea, 100mM NaCl, 50mM Tris.HCl (pH: 7.4), 15 mM β-mercaptoethanol, 2.0mM EDTA
Optimal Storage Temperature
-20 °C to -70 °C (avoid repeated freeze-thawing)

Quality Control Methods / Results

Protein % Purity Range
≥ 98%
Quality Methods Used
SDS PAGE: Major ~21-22 kDa band
Total Protein Method
Bradford Protein Assay vs. BSA as reference standard

Physical Characteristics

Shelf Life
5 years @ -25 °C or ↓
Filtration
No filtration

Troponin-I is one of three subunits that form the troponin complex of the thin filaments of striated muscle.  The troponin complex serves as a calcium-sensitive switch that regulates striated muscle contraction.  Troponin-I binds actin and inhibits actomyosin ATPase activity in the abscence of calcium.  Skeletal muscle Troponin-I exists in two isoforms, TnI-fast and TnI-slow, expressed respectively in fast- and slow-twitch muscle fibers.  The two isoforms are characterized by molecular weights ~21 kDa (fast) and ~22 kDa (slow).  The ratios of slow and fast isoforms vary with muscle source and species.  Skeletal muscle Tn-I offered by TriChem Resources is a mixture of slow and fast isoforms.  It is purified by ion-exchange chromatography in conjunction with calcium dependent affinity chromatography on Troponin-C agarose.

 

HAZARD TESTING & WARNINGS:

Blood samples from tissue donors used in the manufacture of this product tested negative for HBsAg, Anti-HIV-1/HIV-2, & anti-HCV by FDA approved methods.

 

CAUTION:

NO TEST CAN GUARANTEE A PRODUCT TO BE NON-INFECTIOUS.  THEREFORE, ALL MATERIAL DERIVED FROM HUMAN FLUIDS OR TISSUES SHOULD BE CONSIDERED AS POTENTIALLY INFECTIOUS.

 

Use Statement: For Research, Remanufacturing, or Immunization use for products not subject to license under PHS Act Section 351 only.