Human Cardiac Troponin-I (Recombinant)

Product Code

11033E

Stage / Grade

Highly Purified / Recombinant

R.M. Source

Expressed in E. coli

Concentration Range

0.5 - 4.0 mg/mL

Buffer Matrix & pH

6M Urea, 50mM Tris (pH 8.0)

Optimal Storage Temperature

-20 °C to -70 °C (avoid repeated freeze-thawing)

Quality Control Methods / Results

Protein Quantitation Method

Bradford Protein Assay vs. BSA as reference standard

Protein % Purity Range

≥ 98% Pure

Quality Methods Used

SDS PAGE: Major 24 kDa band
Western Blot: Identity confirmed using anti-human cardiac Troponin-I monoclonal antibody.

Physical Characteristics

Shelf Life

5 years @ -25 °C or ↓

Filtration

No filtration

Troponin-I (Tn-I) is one of three sub-units that form the troponin complex of the thin filaments of striated muscle. The troponin complex serves as a calcium-sensitive switch that regulates striated muscle contraction. Tn-I binds actin and inhibits actomyosin ATPase activity in the absence of calcium. Cardiac Tn-I is characterized by a molecular weight of ~24 kDa. It is expresssed only in the heart and has proved to be a specific biomarker for heart disease. Recombinant cardiac Tn-I is purified from E. coli extracts by ion-exchange chromatography in conjunction with calcium dependent affinity chromatography on troponin-C agarose. Recombinant human cardiac troponin-I is recognized by antibodies targeted against native human cardiac troponin-I.

HAZARD TESTING & WARNINGS:

N/A

Use Statement: For Research, Remanufacturing, or Immunization use for products not subject to license under PHS Act Section 351 only.