Troponin-I (Tn-I) is one of three sub-units that form the troponin complex of the thin filaments of striated muscle. The troponin complex serves as a calcium-sensitive switch that regulates striated muscle contraction. Tn-I binds actin and inhibits actomyosin ATPase activity in the absence of calcium. Cardiac Tn-I is characterized by a molecular weight of ~24 kDa. It is expresssed only in the heart and has proved to be a specific biomarker for heart disease. Recombinant cardiac Tn-I is purified from E. coli extracts by ion-exchange chromatography in conjunction with calcium dependent affinity chromatography on troponin-C agarose. Recombinant human cardiac troponin-I is recognized by antibodies targeted against native human cardiac troponin-I.
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