Human Cardiac Troponin-I (Recombinant)

Human Cardiac Troponin-I (Recombinant)
Product Code
Stage / Grade
Highly Purified / Recombinant
R.M. Source
Expressed in E. coli
Concentration Range
0.5 - 4.0 mg/mL
Buffer Matrix & pH
6M Urea, 50mM Tris (pH 8.0)
Optimal Storage Temperature
-20 °C to -70 °C (avoid repeated freeze-thawing)

Quality Control Methods / Results

Protein Quantitation Method
Bradford Protein Assay vs. BSA as reference standard
Protein % Purity Range
≥ 98% Pure
Quality Methods Used
SDS PAGE: Major 24 kDa band
Western Blot: Identity confirmed using anti-human cardiac Troponin-I monoclonal antibody.

Physical Characteristics

Shelf Life
5 years @ -25 °C or ↓
No filtration

Troponin-I (Tn-I) is one of three sub-units that form the troponin complex of the thin filaments of striated muscle.  The troponin complex serves as a calcium-sensitive switch that regulates striated muscle contraction.  Tn-I binds actin and inhibits actomyosin ATPase activity in the absence of calcium.  Cardiac Tn-I is characterized by a molecular weight of ~24 kDa.  It is expresssed only in the heart and has proved to be a specific biomarker for heart disease.  Recombinant cardiac Tn-I is purified from E. coli extracts by ion-exchange chromatography in conjunction with calcium dependent affinity chromatography on troponin-C agarose.  Recombinant human cardiac troponin-I is recognized by antibodies targeted against native human cardiac troponin-I.





Use Statement: For Research, Remanufacturing, or Immunization use for products not subject to license under PHS Act Section 351 only.