Troponin-I (Tn-I) is one of three subunits that form the troponin complex of the thin filaments of striated muscle. The troponin complex serves as a calcium-sensitive switch that regulates striated muscle contraction. Tn-I binds actin and inhibits actomyosin ATPase activity in the absence of calcium. Cardiac Tn-I is characterized by a molecular weight of ~24 kDa. It is expresssed only in the heart and has proved to be a specific biomarker for cardiac muscle damage. Cardiac Tn-I is purified by ion-exchange chromatography in conjunction with calcium dependent affinity chromatography on Troponin-C agarose.
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