Rabbit Skeletal Muscle Troponin-I

Rabbit Skeletal Muscle Troponin-I
Product Code
Stage / Grade
Highly Purified
R.M. Source
Fresh Frozen Rabbit Skeletal Muscle
Concentration Range
0.5 - 2.0 mg/mL
Buffer Matrix & pH
8M Urea, 50mM TrisCl (pH 7.4), 100mM NaCl, 1mM EDTA, 15mM β-mercaptoethanol
Optimal Storage Temperature
-20 °C to -70 °C

Quality Control Methods / Results

Protein % Purity Range
≥ 95%
Quality Methods Used
SDS PAGE: Major ~21-22 kDa band

Physical Characteristics

Shelf Life
5 years @ -25 °C or ↓
No filtration

Troponin-I is one of three subunits that form the troponin complex of the thin filaments of striated muscle.  The troponin complex serves as a calcium-sensitive switch that regulates striated muscle contraction.  Tn-I binds actin and inhibits actomyosin ATPase activity in the abscence of calcium.  Skeletal muscle Tn-I exists in two isoforms, TnI-fast and TnI-slow, expressed respectively in fast and slow-twitch muscle fibers.  The two isoforms are characterized by molecular weights of ~21 kDa (fast) and ~22 kDa (slow).  The ratios of slow and fast isoforms vary with muscle source and species.  Skeletal muscle Tn-I offered by TriChem Resources is likely a mixture of slow and fast isoforms.  It is purified by ion-exchange chromatography in conjunction with calcium dependent affinity chromatography on Troponin-C agarose.





Use Statement: For use in Research or Remanufacture of non-injectable products  not subject to license under PHS Act Section 351 only.